Objective: To create an in-vitro model for raised intraocular pressure by exposing lamina cribrosa cells to cyclical mechanical stretch and examine the effect of this stimulus on gene expression patterns of components and modulators of the extracellular matrix (ECM) of the lamina cribrosa. Methods: Confluent normal primary human lamina cribrosa were transferred onto flexible Objective: To create an in-vitro model for raised intraocular pressure by exposing lamina cribrosa cells to cyclical mechanical stretch and examine the effect of this stimulus on gene expression patterns of components and modulators of the extracellular matrix (ECM) of the lamina cribrosa. Methods : Confluent normal primary human lamina cribrosa were transferred onto flexible