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从发病的杂交鲍(皱纹盘鲍×盘鲍)稚鲍中共分离27株菌株,并对其分泌胞外产物(包括酪蛋白酶、明胶酶、淀粉酶、脂肪酶、磷脂酶和溶血素)的能力进行了分析。试验结果表明,具有分泌胞外蛋白酶、明胶酶、淀粉酶、脂肪酶、磷脂酶和溶血素能力的菌株分别占总菌株数的81.5%(22/27)、74.1%(20/27)、18.5%(5/27)、0%(0/27)、11.1%(3/27)、29.6%(8/27)。其中,1#、14#菌株具有很强的产胞外蛋白酶能力,而明胶酶的最大分泌者为2#、7#、9#、17#菌株,淀粉酶的为11#、14#菌株,磷脂酶的为11#、15#菌株,溶血素的为菌株10#、21#。此外,10#、11#、15#菌株可同时分泌4种胞外产物,而12#、14#、18#、21#、23#、24#菌株可同时分泌3种胞外产物。综合考虑每一种胞外酶的潜在致病作用,以及菌株分泌多种胞外酶的能力,初步确认11株菌为潜在的致病菌:1#、2#、7#、9#、10#、11#、12#、14#、15#、17#、21#。用API条带法对它们进行了种类鉴定,11株菌中巴斯德氏菌和假单胞菌所占的比例分别为45.5%、27.3%。
Seventy-seven strains were isolated from diseased hybrid abalone (Abalone abalone × abalone) and their ability to secrete extracellular products including casein, gelatinase, amylase, lipase, phospholipase and hemolysin Analyzed. The results showed that the strains with the ability to secrete extracellular protease, gelatinase, amylase, lipase, phospholipase and hemolysin accounted for 81.5% (22/27), 74.1% (20/27), 18.5 % (5/27), 0% (0/27), 11.1% (3/27), 29.6% (8/27). Among them, strain # 1 and strain # 14 had a strong ability to produce extracellular protease, while gelatinase was the largest secreted by strains 2 #, 7 #, 9 # and 17 #, and amylase was 11 # and 14 # Phospholipase 11 #, 15 # strains, hemolysin strain 10 #, 21 #. In addition, 10 #, 11 # and 15 # strains could secrete 4 kinds of extracellular products simultaneously, while 12 #, 14 #, 18 #, 21 #, 23 # and 24 # strains could simultaneously secrete three kinds of extracellular products. Considering the potential pathogenicity of each extracellular enzyme and the ability of the strain to secrete various extracellular enzymes, 11 strains of bacteria were identified as potential pathogens: 1 #, 2 #, 7 #, 9 #, 10 #, 11 #, 12 #, 14 #, 15 #, 17 #, 21 #. They were identified by API banding method. The proportion of Pasteurella and Pseudomonas in 11 strains was 45.5% and 27.3% respectively.