论文部分内容阅读
为了观察IL-18在BXSB狼疮性肾炎小鼠的动态表达,并探讨IL-18在LN发生发展中的作用,将C57BL/6小鼠(正常对照)和BXSB小鼠(LN模型)各24只分别随机分为4组,观察至10、12、16、20周,PASM染色法观察不同周龄小鼠肾组织病理变化,直接免疫荧光法检测肾组织IgG的沉积,ELISA法检测小鼠血清IL-18水平,RT-PCR法检测肾组织和脾组织IL-18 mRNA表达水平,免疫组化法检测肾组织IL-18表达水平,并分析LN小鼠系统和肾脏IL-18表达与肾组织病理和免疫病理的相关性。结果显示,LN组小鼠肾组织IgG沉积随时间进展逐渐增多,肾组织病理损伤评分、血清IL-18水平和肾小管IL-18表达量在12周后进行性升高,肾小球IL-18表达量在16周后进行性升高,且均较正常对照组明显升高(P<0.05);不同周龄LN小鼠肾组织和脾组织IL-18 mRNA表达与正常对照组之间无统计学差异,LN小鼠血清IL-18水平、肾小球及肾小管IL-18表达量与肾小球损伤评分及肾组织IgG沉积评分呈正相关。推测IL-18可能在LN发生发展中发挥重要作用,且转录后过程对于系统和肾脏局部IL-18的高表达起主要作用。
In order to observe the dynamic expression of IL-18 in BXSB lupus nephritis mice and to explore the role of IL-18 in the development and progression of LN, C57BL / 6 mice (normal control) and BXSB mice (LN model) The rats were randomly divided into 4 groups and observed at 10, 12, 16, and 20 weeks. PASM staining was used to observe the pathological changes of kidney in different age mice. The deposition of IgG in renal tissue was detected by immunofluorescence method. IL-18 levels in renal and spleen tissues were detected by RT-PCR. The expression of IL-18 in renal tissues and spleen tissues was detected by immunohistochemistry. The expression of IL-18 in renal tissues and kidneys was analyzed by immunohistochemistry. Correlation with immunopathology. The results showed that the deposition of IgG in renal tissue of LN mice gradually increased with time, the pathological damage scores of renal tissue, serum IL-18 levels and tubular IL-18 expression increased progressively after 12 weeks, 18 in LN group were significantly higher than those in normal control group after 16 weeks (P <0.05). The expression of IL-18 mRNA in renal tissue and spleen tissue of LN mice at different weeks was not significantly different from that in normal control group Statistically, the level of IL-18 in serum of LN mice and the expression of IL-18 in glomeruli and tubules were positively correlated with glomerular injury score and IgG deposition rate in kidney tissue. It is speculated that IL-18 may play an important role in the development of LN, and the post-transcriptional process plays a major role in the high expression of local IL-18 in the system and the kidney.