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目的探讨米胚芽对成年小鼠糖耐量的影响及作用机制。方法 6周龄ICR小鼠30只,按体重随机分为对照组、低、高剂量米胚芽组,分别给予基础饲料、添加10%和40%米胚芽饲料饲养4周,实验结束后测空腹血糖、胰岛素,计算胰岛素敏感指数(ISI),进行口服葡萄糖耐量试验(OGTT);取小鼠血清和肝组织测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛含量;采用荧光实时定量PCR(RT-PCR)测定小鼠肝脏组织中葡萄糖激酶(GCK)和脂肪组织中葡萄糖转运体-4(GLUT-4)mRNA表达水平。结果与对照组比较,高剂量米胚芽组小鼠ISI(-2.59±0.33)明显升高、0.5 h血糖[(6.75±2.20)mmol/L]与血糖曲线下面积(12.81±3.19)下降(P<0.05);与对照组比较,高剂量米胚芽组小鼠肝脏组织中GSH-Px、SOD酶活力[分别为(44.34±11.96)、(34.88±7.70)U/gprot]升高,丙二醛含量[(1.57±0.43)nmol/m L]降低;与对照组比较,低、高剂量米胚芽组小鼠血清中SOD活力[分别为(48.45±2.54)、(53.27±2.51)U/m L]升高,MDA含量[分别为(1.44±0.25)、(1.46±0.35)nmol/m L]降低(P均<0.05);与对照组比较,高剂量米胚芽组小鼠肝脏组织GCK mRNA表达水平上调,低高剂量米胚芽组小鼠脂肪组织GLUT-4 mRNA表达水平上调(P<0.05)。结论米胚芽可提高成年小鼠的糖耐量水平,其机制可能与其抗氧化活性和调控糖代谢相关基因表达有关。
Objective To investigate the effect of rice germ on glucose tolerance in adult mice and its mechanism. Methods Thirty 6-week-old ICR mice were randomly divided into control group, low and high dose rice germ groups and fed with basal diet supplemented with 10% and 40% germ feed for 4 weeks. After the experiment, fasting blood glucose (ISI), and oral glucose tolerance test (OGTT). The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured in serum and liver of mice, Vitality and malondialdehyde (MDA) content were measured. The mRNA expression of GLUT-4 in glucokinase (GCK) and adipose tissue was detected by real-time quantitative PCR (RT- Results Compared with the control group, the ISI of high-dose rice germ group was significantly increased (-2.59 ± 0.33), the hypoglycemia (6.75 ± 2.20 mmol / L) and the area under the curve of blood glucose (12.81 ± 3.19) <0.05). Compared with the control group, the activities of GSH-Px and SOD in the liver tissue of high-dose rice germ group [(44.34 ± 11.96) and (34.88 ± 7.70) U / gprot, respectively] (1.57 ± 0.43) nmol / m L]. Compared with the control group, the activity of SOD in serum of mice in low and high dose rice germ groups [(48.45 ± 2.54) and (53.27 ± 2.51) U / m L, respectively] ] And MDA content [(1.44 ± 0.25) and (1.46 ± 0.35) nmol / m L, respectively] (all P <0.05). Compared with the control group, the GCK mRNA expression in the liver of high-dose rice germ group The levels of GLUT-4 mRNA were up-regulated in adipose tissue of low-dose rice germ group (P <0.05). Conclusion Rice germ can increase the level of glucose tolerance in adult mice, and its mechanism may be related to its antioxidant activity and regulation of gene expression related to glucose metabolism.