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目的:探讨1,6-二磷酸果糖(FDP)对脓毒症大鼠凝血功能及炎症因子的影响。方法:依据随机数字表,60只健康成年雄性大鼠分成6组,分别为生理盐水对照组(NSc)、CLP组(经典盲肠结扎穿孔术)、FDP组(FDP0.33、FDP1.65、FDP3.30)以及FDP对照组(FDPc)。利用经典盲肠结扎穿孔术(cecal ligation and puncture,CLP)构建脓毒症大鼠模型。生理盐水对照组(NSc)行假手术加生理盐水输注。CLP组行CLP术加生理盐水输注。FDP组采用CLP加不同浓度的FDP输注;根据输入浓度的不同,分为3组,分别输注FDP浓度0.33g/kg(FDP0.33),1.65g/kg(FDP1.65),3.30g/kg(FDP3.30)。FDP对照组(FDPc)行假手术加FDP输入,浓度为3.30g/kg。各组液体输入时间为CLP术后3h。术后6h及12h分别采血测各组大鼠血浆肿瘤坏死因子α(TNF-α)、白介素6(IL-6)及凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(Fib)及血栓弹力图(TEG)等。比较各组大鼠各时间点生存率,时间点分别为6h、12h、24h及48h。结果:同生理盐水对照组(NSc)相比,CLP组及FDP组(FDP0.33、FDP1.65、FDP3.30)术后6h、12h大鼠血浆TNF-α、IL-6显著升高(P<0.01)。FDP各组(FDP0.33、FDP1.65、FDP3.30)TNF-α、IL-6浓度显著低于CLP组(P<0.05)。术后6h及12hFDP1.65与FDP3.30组中PT、APTT、Fib显著低于CLP组(P<0.05),FDP0.33组与CLP组差异无统计学意义(P>0.05)。术后6h及12hFDP各组(FDP0.33、FDP1.65、FDP3.30)R、K值显著低于CLP组(P<0.05)。术后6h及12hFDP1.65组及FDP3.30组MA值显著低于CLP组(P<0.05)。各组α及CI值在各时间点差异无统计学意义(P<0.05)。各组大鼠48h生存率差异无统计学意义(P>0.05)。结论:FDP能抑制脓毒症大鼠炎症反应并在早期能改善脓毒症大鼠凝血功能。
Objective: To investigate the effect of fructose-1,6-diphosphate (FDP) on the coagulation function and inflammatory cytokines in septic rats. Methods: According to the random number table, 60 healthy adult male rats were divided into 6 groups: saline control group (NSc), CLP group (classic cecal ligation and perforation), FDP group (FDP0.33, FDP1.65, FDP3 .30) and FDP control group (FDPc). A classic rat model of sepsis was established by cecal ligation and puncture (CLP). Saline control group (NSc) sham operation plus saline infusion. CLP group with CLP plus saline infusion. FDP group was treated with CLP plus different concentrations of FDP infusion. According to the different input concentrations, the rats were divided into 3 groups: FDP 0.33g / kg (FDP 0.33), 1.65g / kg (FDP 1.65), 3.30g /kg(FDP3.30). FDP control group (FDPc) sham operation plus FDP input, the concentration of 3.30g / kg. Each group of liquid input time 3h after CLP. Blood samples were collected at 6 and 12 hours after operation to measure the levels of plasma TNF-α, IL-6, PT, APTT, Original (Fib) and Thromboelastography (TEG) and so on. The survival rates of rats in each group were compared at 6h, 12h, 24h and 48h respectively. Results: The levels of plasma TNF-α and IL-6 in CLP group and FDP group (FDP0.33, FDP1.65, FDP3.30) at 6h and 12h after operation were significantly higher than those in control group (NSc) P <0.01). The concentrations of TNF-α and IL-6 in FDP groups (FDP 0.33, FDP 1.65, FDP3.30) were significantly lower than those in CLP group (P 0. 05). The PT, APTT and Fib levels in FDP1.65 and FDP3.30 groups at 6h and 12h after operation were significantly lower than those in CLP group (P <0.05). There was no significant difference between FDP0.33 group and CLP group (P> 0.05). The R and K values of FDP 0.33, FDP 1.65 and FDP3.30 at 6 and 12 h after operation were significantly lower than those in CLP group (P <0.05). MA values in FDP1.65 group and FDP3.30 group at 6h and 12h after operation were significantly lower than those in CLP group (P <0.05). There was no significant difference of α and CI in each group at all time points (P <0.05). There was no significant difference in the 48-h survival rate of rats in each group (P> 0.05). Conclusion: FDP can inhibit inflammatory response in septic rats and improve coagulation function in septic rats in early stage.