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作者将２型登革（ＤＥＮ－２）病毒的Ｅ基因克隆于穿梭载体ｐＶＬ－９４１－ｐｏｌｙ，通过在草地夜蛾细胞内同源重组将重组载体转染到苜蓿银纹夜蛾ＲＰ２３－ｌａｃＺ基因组中，从细胞上清提取的重组Ｅ蛋白用ＴＡＬＯＮＴＭ树脂纯化后制成重组疫苗，与ＤＥＮ－２ ＰＤＫ５３
In this study, we cloned the E gene of type 2 dengue (DEN-2) virus into the shuttle vector pVL-941-poly and transfected it into the RP23-lacZ genome of Autographa californica by homologous recombination in S. frugiperda cells , The recombinant E protein extracted from the cell supernatant was purified with TALONTM resin and made into a recombinant vaccine, which was used in combination with DEN-2 PDK53