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目的分析肺结核患者(TB)外周血单核细胞中miR-34a-5p和miR-708-5p的表达水平,并探讨其临床诊断价值。方法选取TB患者和健康对照(HD)各25例,应用Taq Man q PCR技术测定两组人群外周血单核细胞中miR-34a-5p和miR-708-5p表达水平,以U6 sn RNA作为内参。应用ROC曲线评价miR-34a-5p和miR-708-5p诊断TB的临床意义。结果 miR-34a-5p在TB患者中的表达水平为(10.08±0.68),显著高于HD的(7.67±0.36),差异有统计学意义(P<0.01);miR-708-5p在TB患者中的表达水平显著高于HD的(13.52±0.98 Vs 7.71±0.57),差异有统计学意义(P<0.01)。ROC曲线分析表明在肺结核诊断中,miR-34a-5p的敏感性、特异性分别为72%、76%;miR-708-5p的敏感性、特异性为76%、76%。结论 miR-34a-5p和miR-708-5p分子在TB患者外周血单核细胞中异常表达,对TB辅助诊断具有一定参考价值。
Objective To analyze the expression of miR-34a-5p and miR-708-5p in peripheral blood mononuclear cells of patients with tuberculosis (TB), and to explore its clinical value. Methods Twenty-five patients with TB and healthy controls (HD) were selected. The expression of miR-34a-5p and miR-708-5p in peripheral blood mononuclear cells was detected by Taq Man q PCR. U6 sn RNA was used as internal reference . To evaluate the clinical significance of miR-34a-5p and miR-708-5p in the diagnosis of TB using ROC curve. Results The expression level of miR-34a-5p in TB patients was significantly higher than that in HD patients (10.08 ± 0.68 vs 7.67 ± 0.36, P <0.01) (13.52 ± 0.98 vs 7.71 ± 0.57), the difference was statistically significant (P <0.01). ROC curve analysis showed that the sensitivity and specificity of miR-34a-5p were 72% and 76%, respectively. The sensitivity and specificity of miR-708-5p were 76% and 76%, respectively. Conclusion The abnormal expression of miR-34a-5p and miR-708-5p in peripheral blood mononuclear cells of patients with TB may be of reference value in the diagnosis of TB.