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通过对2009-2010年河北省145株及外省30株马铃薯早疫病菌对代森锰锌的敏感性、产孢量两个表型性状和AFLP基因型的测定,揭示了河北省早疫病菌群体的遗传结构。毒力测定表明所有供试菌株对代森锰锌均表现敏感,其EC50范围介于1.04-4.86μg/mL之间,平均为2.93μg/mL。被测河北省47株早疫病菌产孢量平均为85个孢子/mm2,大大高于30个对照菌株的产孢量,对照菌株的平均产孢量为50个孢子/mm2,菌株间产孢量存在明显差异。AFLP聚类分析揭示出了马铃薯早疫病菌丰富的遗传多样性,每个菌株都具有独特的AFLP基因型。早疫病菌AFLP基因型与地理来源存在着密切的相关性,与菌株产孢量有一定的相关性,但与代森锰锌敏感性无相关性。
The results showed that the susceptibility of A. tomentosa to mancozeb and the two phenotypic traits of sporulation and AFLP genotypes in 145 isolates from Hebei Province in 2009-2010 and from other provinces in China revealed that the population of A. frigotophilus The genetic structure. Toxicity tests showed that all the tested strains were sensitive to mancozeb with EC50 ranging from 1.04-4.86μg / mL with an average of 2.93μg / mL. The measured sporulation of 47 A. oriforms in Hebei Province was 85 spores / mm2 on average, which was much higher than that of 30 control spores. The average sporulation of the control strains was 50 spores / mm2. Significant differences exist. AFLP clustering analysis revealed a rich genetic diversity of A. solani, each with a unique AFLP genotype. The AFLP genotypes of A. blight were closely related to geographical origin, and had some correlation with sporulation of strains, but not with the sensitivity of mancozeb.