通过花粉管导入法将新疆大赖草（Ｌｅｙｍｕｓ　ｒａｃｅｍｏｓｕｓ（Ｌａｍ．）Ｔｚｖｅｌ）供体ＤＮＡ导入春小麦７６１（Ｔｒｉｔｉｃｕｍ　ａｅｓｔｉｖｕｍ）受体的胚囊中，获得了大穗及密穗两大类遗传变异后代。经用改进的酸性聚丙烯酸胺凝胶电脉法（Ａ－ＰＡＧＥ），分析了１０个株系样品的种子醇溶蛋白，图谱结果显示５个大穗变异株系样品之间表现为同样带型，均与受体有显著差异，表现为某些带的消失与增加，而且增加带与供体的电泳条带位置相同；４个密穗变异株系样品和受体相比有两种带型，表现为带的减少与浅带的加深。这些均表明大救草ＤＮＡ以花粉管为通道导入小麦，转化株系后代中发生了基因水平的变异。 The donor DNA of Leymus racemosus (Lam.) Tzvel was introduced into the embryo sac of Triticum aestivum by pollen tube induction method, and two major genetic variations, large panicle and dense panicle, were obtained. The prolamins of ten strains were analyzed by modified acidic polyacrylamide gel electrophoresis (A-PAGE), and the results showed that the samples of the five big-spike strains showed the same type , Which were significantly different from that of the recipient, showing the disappearance and increase of some bands, and the bands of the bands with the donor were the same. The electrophoresis bands of the four mutant strains had two bands , Manifested as a decrease of the band and shallow deepening. All these showed that the DNA of the paralyzed grass was introduced into wheat through the pollen tube, and genetic variation occurred in the progeny of the transformed lines.