引物是决定ＰＣＲ结果的关键。本实验中引物１、２虽有２６个碱基，但由于其５端有６个碱基为限制性内切酶的部分识别序列，所以实际上只有２０个碱基与原始模板互补配对。采用标准的ＰＣＲ方法不能产生理想的结果。经采用ＰＣＲ－两段法从喉癌组织中扩增得到４７７ｂｐ的ＤＮＡ片段－ｎｍ２３－Ｈ１基因。对于应用ＰＣＲ技术扩增人类基因组基因做了有益的尝试，扩增的ｎｍ２３－Ｈ１基因为进一步做变异分析打下基础 Primers are the key to determining PCR results. In this experiment, primers 1 and 2, although having 26 bases, had only 6 bases in its 5 termini for the partial recognition sequence of the restriction enzyme, so only 20 bases were actually paired with the original template. Using standard PCR methods does not produce the desired results. The 477bp DNA fragment-nm23-H1 gene was amplified from laryngeal carcinoma by PCR-two-stage method. It is a useful attempt to amplify the human genome by PCR, and the amplified nm23-H1 gene lays the foundation for further mutation analysis