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目的:探讨汉防己甲素防治肝纤维化的机理。方法:用四氯化碳诱导SD大鼠肝纤维化,同时给予汉防己甲素治疗,对照组给予生理盐水。观察不同实验阶段动物肝组织中Ⅳ型胶原及含结蛋白细胞的变化,并用电镜观察储脂细胞的形态变化。结果:治疗组肝纤维化程度明显轻于对照组。治疗组肝组织中储脂细胞增殖及转化受到抑制,Ⅳ型胶原沉积减少,结蛋白阳性细胞数明显少于对照组(P<0.01)。结论:汉防己甲素能有效地治疗肝纤维化,其机理在于抑制储脂细胞的增殖及转化,减少Ⅳ型胶原在肝组织中沉积。
Objective: To investigate the mechanism of tetrandrine against hepatic fibrosis. Methods: Liver fibrosis was induced by carbon tetrachloride in rats, meanwhile tetrandrine was given to the rats. The control group was given normal saline. The changes of type Ⅳ collagen and protein in rat liver tissue were observed at different experimental stages. The morphological changes of the lipid-storing cells were observed under electron microscope. Results: The degree of liver fibrosis in the treatment group was significantly lower than that in the control group. In the treatment group, the proliferation and transformation of lipid-storing cells in the liver tissue were inhibited, the type Ⅳ collagen deposition was reduced, and the number of positive cells in the treated group was significantly less than that in the control group (P <0.01). Conclusion: Tetrandrine can effectively treat hepatic fibrosis. Its mechanism is to inhibit the proliferation and transformation of lipid-storing cells and reduce the deposition of type Ⅳ collagen in liver tissue.