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目的 分析麝香对退变椎间盘基因表达谱的调控作用。方法 建立颈椎间盘退变模型。从 9个月模型组、麝香治疗组大鼠颈椎间盘中抽提mRNA ,经反转录分别用Cy3、Cy5荧光标记 ,获得椎间盘cDNA的探针 ;cDNA探针与大鼠BiostarR - 4 0s基因表达谱芯片杂交 ,结果由激光扫描仪扫描并用软件进行图像分析、标准化处理、ratio值分析、聚类分析。结果 麝香组与模型组比较 ,11.1% (共 4 4 1条 )基因表达发生明显变化 ,其中 2 6 0条基因表达量上升(R >2 .0 ) ,182条基因表达量明显下降 (R <0 .5 )。经过信息学分析 ,在上述两张芯片上都有基因表达量明显变化的 90条 (R >2 .0或 <0 .5 ) ,其中基因表达量都明显上调的 2 5条 (R >2 .0 ) ,其中功能明确的基因有 3条 ;都明显下调的 2 0条 (R <0 .5 ) ,其中功能明确的基因有 5条 ;麝香发挥明显上调作用的共 13条 (由R <0 .5到R >2 .0 )。结论 麝香对退变椎间盘出现总体调控。筛选出大鼠退变椎间盘中相关基因 ,特别是有较多的细胞信号和传递蛋白类基因表达 ,说明细胞内外的信号传导介导了椎间盘退变过程。
Objective To analyze the regulation of musk on the gene expression profile of degenerated intervertebral discs. Methods The cervical disc degeneration model was established. mRNA was extracted from the cervical intervertebral discs of 9-months model group and musk-treated group and labeled with Cy3 and Cy5 by reverse transcription to obtain cDNA probes for the intervertebral disc; cDNA probes and Rat BiostarR - 40s gene expression Spectral chip hybridization results were scanned by a laser scanner and analyzed by software for image analysis, normalization, ratio analysis, and cluster analysis. Results Compared with the model group, 11.1% (a total of 441) gene expression changed significantly in the musk group, of which the expression of 260 genes increased (R>2. 0), and the expression of 182 genes decreased significantly (R < 0 .5 ). After informatics analysis, there were 90 significant changes in gene expression on both chips (R > 2.0 or <0.5), of which 25 were significantly upregulated (R > 2). 0) , of which there are 3 genes with well-defined functions; 20 are significantly down-regulated (R < 0.5), of which there are 5 genes with well-defined functions; 13 of them are obviously up-regulated (by R <0 .5 to R > 2. 0 ). Conclusion Musk has overall control on degenerative discs. The relevant genes in the degenerated intervertebral discs of rats were screened, in particular, there were more cell signal and transmission protein gene expressions, indicating that signal transduction inside and outside the cell mediates the degeneration of intervertebral discs.