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The 3.1 kb BamHI fragment containing the chloroplast rbcL gene from grape (Vitis vinifera L) hasbeen cloned and its restriction map and nucleotide sequence determined. The complete nucleotidesequence is 20O4 bp long with a coding sequence of 1428 bp, which cncode a polypeptide of 475amino acids with a predicted molecular weight of 53 kd. The 5’ upstream sequence including theputative promoter is 358 bp, with a -10 sequence (TAAAAT), a -35 sequence (TTGCGC) and theSD sequence (GGAGG). The 218 bp long 3’ downstream sequence contains three transcription stem-loop tendnation structures. The homologies of this gene with those of tobacco, petunia, spinach,alfalfa, rice and maise are 91.5%, 91.4% 90.2%, 89.8%, 86.3% and 84.5% respectively while thehomologies between their putative polypeptide sequences are 92.2% 91.6%, 92.2%, 93.7%, 93.5%and 90.1% respectively.
The 3.1 kb BamHI fragment containing the chloroplast rbcL gene from grape (Vitis vinifera L) hasbeen cloned and its restriction map and nucleotide sequence determined. The complete nucleotide sequence is 20O4 bp long with a coding sequence of 1428 bp, which cncode a polypeptide of 475 amino acids with a predicted molecular weight of 53 kd. The 5 ’upstream sequence includes the exportative promoter is 358 bp with a -10 sequence (TAAAAT), a-35 sequence (TTGCGC) and the SD sequence (GGAGG). The 218 bp long 3’ The homologies of this gene with those of tobacco, petunia, spinach, alfalfa, rice and maize are 91.5%, 91.4% 90.2%, 89.8%, 86.3% and 84.5% respectively while thehomologies between their putative polypeptide sequences are 92.2% 91.6%, 92.2%, 93.7%, 93.5% and 90.1% respectively.