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本文采用正交设计,初步探讨了厌氧棒菌苗(CP)诱导内源性肿瘤坏死因子(TNF)活性的条件。结果表明:仅用CP一种调节剂,按照一定方式对小鼠进行三次刺激.便可使其血清具有显著的内源性TNF活性。有TNF活性的部分经SDS—PAGE和凝胶过滤测得分子量分别约为60,000dal和55,000dal;醋酸纤维膜电泳显示为α球蛋白;TNF活性经56℃处理30分钟仍稳定,70℃,10分钟即丧失;在pH6～9之间、活性稳定。
In this paper, orthogonal design was used to investigate the conditions of anaerobic bacilli (CP) -inducing the activity of endogenous tumor necrosis factor (TNF). The results showed that: only a regulator of CP, according to a certain manner in mice three times to stimulate its serum can have significant endogenous TNF activity. The fraction with TNF activity was about 60,000dal and 55,000dal, respectively, as determined by SDS-PAGE and gel filtration; the acetate membrane electrophoresis showed alpha globulin; TNF activity was stable at 56 ° C for 30 minutes; 70 ° C, 10 Minute is lost; in pH6 ~ 9, activity is stable.