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目的探讨新生儿缺氧缺血性脑损伤时血清硫化氢(H2S)水平的变化情况。方法选取本院儿科2004年12月至2009年9月因缺氧缺血性脑病或颅内出血住院的新生儿为观察组,同期因怀疑咽下综合征入院、最终排除各种疾病的新生儿20例为对照组。观察组分别于生后1~2天(急性期)及7~10天(恢复期)、对照组于生后1~2天留取空腹静脉血,采用ELISA法检测血清中H2S水平。结果观察组(52例)急性期H2S水平较对照组明显升高[(4.6±2.9)μmol/L比(2.4±1.7)μmol/L,P=0.002],观察组恢复期(31例)H2S水平较急性期(31例)明显降低[(1.7±1.7)μmol/L比(4.6±3.4)μmol/L,P=0.000];观察组恢复期血清H2S水平与对照组比较,差异无统计学意义(P>0.05)。早产儿和足月儿、缺氧缺血性脑病组和颅内出血组H2S水平差异均无统计学意义(P>0.05)。结论新生儿缺氧缺血性脑损伤后血清中H2S水平增高,并随疾病恢复而降低,发生动态变化。
Objective To investigate the changes of serum hydrogen sulfide (H2S) levels in neonates with hypoxic-ischemic brain damage. Methods The neonates admitted to our hospital from December 2004 to September 2009 due to hypoxic-ischemic encephalopathy or intracranial hemorrhage were selected as the observation group. During the same period, neonates who were admitted to hospital for suspected swallowing syndrome were excluded. Example for the control group. The observation group were given 1 to 2 days after birth (acute phase) and 7 to 10 days (recovery phase). The control group were given fasting venous blood 1 to 2 days after birth and the serum H2S levels were measured by ELISA. Results The H2S level in the acute phase of the observation group was significantly higher than that of the control group [(4.6 ± 2.9) μmol / L vs (2.4 ± 1.7) μmol / L, P = 0.002] Compared with the control group, the levels of H2S in the recovery group were significantly lower than those in the control group [(1.7 ± 1.7) μmol / L vs (4.6 ± 3.4) μmol / L, P = Significance (P> 0.05). There was no significant difference in H2S level between preterm and term infants, hypoxic ischemic encephalopathy group and intracranial hemorrhage (P> 0.05). Conclusions The level of H2S in the serum of neonatal hypoxic-ischemic brain injury increases and decreases with the recovery of the disease, with a dynamic change.