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目的:运用血清药理学方法探讨天泰1号含药血清对Aβ_(1-40)诱导PC12 AD细胞模型神经毒性的保护作用及其机制。方法:采用Aβ_(1-40)诱导PC12制备AD细胞模型,给予不同剂量的天泰1号含药血清进行孵育,镜下观察细胞形态学变化,MTT法检测细胞存活率,试剂盒检测细胞SOD活力及MDA含量,免疫荧光染色法观察细胞微管相关蛋白2(MAP-2)的表达,RT-PCR检测促凋亡基因Caspase-12 m RNA表达,Westernblot检测Caspase-12蛋白表达。结果:天泰1号3个不同剂量含药血清(2.5 g/kg/d剂量组、5.0 g/kg/d剂量组和10.0 g/kg/d,剂量组)均显著提高Aβ_(1-40)诱导PC12细胞的存活率,增加SOD活性,减少MDA的释放,下调Caspase-12 m RNA和蛋白的表达。结论:天泰1号通过拮抗氧化应激、促进微管生长、抑制细胞凋亡,保护神经细胞,发挥多靶点多途径抗AD作用。
Objective: To explore the protective effect of tiantai-1 serum on neurotoxicity induced by Aβ 1-40 in PC12 AD cell model and its mechanism by serum pharmacology. Methods: AD12 cells were induced by Aβ 1-40 to induce AD12 cells. Different doses of Tiantai No.1 serum were incubated for morphological changes. Cell viability was detected by MTT assay. The expression of Caspase-12 mRNA was detected by RT-PCR. Caspase-12 protein expression was detected by Western blot. Results: Three different doses of tiantai 1 (2.5 g / kg / d, 5.0 g / kg / d and 10.0 g / kg / d) ) Induced the survival rate of PC12 cells, increased the activity of SOD, reduced the release of MDA and down-regulated the expression of Caspase-12 mRNA and protein. Conclusion: Tiantai 1 can antagonize the oxidative stress, promote the growth of microtubules, inhibit apoptosis, protect neurons and exert multi-target anti-AD effect.