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[目的]观察煤烘高氟玉米染毒大鼠的血清抗酒石酸酸性磷酸酶5b(TRACP-5b)的表达变化,探讨骨吸收在氟骨症发生机制中的作用。[方法]将断乳4周的SD大鼠120只随机分为4组,对照组给予商品化饲料,3个实验组在对照饲料基础上给予不同比例煤烘高氟玉米,低、中、高剂量染毒组饲料含氟量分别为9.56、15.89及23.00mg/kg。于连续染毒至第30、90、180天时,检测大鼠骨氟、尿氟含量及氟斑牙发生率、股骨病理组织学积分,ELISA法检测血清TRACP-5b水平。[结果](1)各剂量组骨氟、尿氟、氟斑牙发生率及股骨病理学积分均随着染毒剂量的增加而升高(P<0.05)。染毒至第30、90、180天时,各剂量组的骨氟、尿氟均高于对照组(P<0.05),且剂量较高的组别的尿氟、骨氟高于剂量较低的组别(P<0.05)。染毒至第90天和180天时,各剂量组氟斑牙发生率高于对照组(P<0.05);中、高剂量组股骨病理切片积分高于低剂量组和对照组(P<0.05)。(2)各组染毒至180天时,血清TRACP-5b均高于同剂量组90天及30天时的水平(P<0.05)。在第30天及90天时,各组血清TRACP-5b差异无统计学意义;染毒至180天时,高剂量组TRACP-5b高于对照组和中剂量组(P<0.05),其余各组间差异无统计学意义。[结论]摄入煤烘高氟玉米剂量较高,且摄入时间较长时,可能引起血清TRACP-5b表达升高。
[Objective] To observe the change of serum tartrate-resistant acid phosphatase 5b (TRACP-5b) expression in coal-fired high fluoride corn-induced rats and explore the role of bone resorption in the pathogenesis of skeletal fluorosis. [Methods] One hundred and twenty SD rats were randomly divided into 4 groups after weaning for 4 weeks, the control group were given commercial feed, and the three experimental groups were given different proportions of coal based on the control feed with high fluoride corn, low, medium and high The dose of fluoride in the dose group was 9.56, 15.89 and 23.00 mg / kg, respectively. At the 30th, 90th, and 180th days after continuous exposure, the content of fluorine and urinary fluoride, the incidence of dental fluorosis, the histopathological score of femur, and the level of TRACP-5b in serum were detected by ELISA. [Results] (1) The incidences of bone fluoride, urinary fluoride and dental fluorosis and the pathological scores of femur in each dose group increased with the increase of exposure dose (P <0.05). At the 30th, 90th and 180th days after exposure, the bone fluoride and urinary fluoride in each dose group were higher than those in the control group (P <0.05), and the urinary fluoride and bone fluoride in the higher dose group were higher than those in the lower dose group Group (P <0.05). The incidence of dental fluorosis in each dose group was higher than that in the control group (P <0.05) after 90 days and 180 days exposure. The pathological sections of middle and high dose group were higher than the low dose group and the control group (P <0.05) . (2) The levels of TRACP-5b in serum of each group were higher than that of the same dose group at 90 and 30 days (P <0.05). At 30 days and 90 days, TRACP-5b in each group had no significant difference. TRACP-5b in high-dose group was higher than that in control group and middle-dose group (P <0.05) The difference was not statistically significant. [Conclusion] The high dose of fluoride corn in the coal bake and the prolonged intake may result in the increase of serum TRACP-5b expression.