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目的了解辽宁省锦州市啮齿动物中鼠种构成、带毒率、汉坦病毒(HV)流行情况及病毒分型。方法采用间接免疫荧光法(IFA)检测鼠肺中HV抗原;用逆转录聚合酶链反应(RT-PCR)法扩增阳性样品中的部分M片段;构建系统发生树进行系统发生分析及分型。结果 2008—2013年锦州市出血热疫区捕鼠1 838只,分属2科4属5种,病毒携带率为4.24%(78/1 838);各年之间鼠肺带毒率有统计学意义(χ2=13.579,P=0.019,P<0.05);部分M片段序列分析表明,11株病毒与汉城病毒(SEOV)核苷酸序列同源性为96.2%~100%,而与汉滩型病毒(HTNV)同源性仅为70.6%~71.2%,系统进化树显示检测阳性的11株SEO起源相同,与Bj HD01株病毒的亲缘关系最近。结论锦州地区HV优势毒株为SEO型,变异较小,基因型别较为稳定,并有明显的地区聚集性。
Objective To understand the rodent species composition, virulence rate, prevalence of Hantavirus (HV) and virus typing in Jinzhou City, Liaoning Province. Methods Indirect immunofluorescence (IFA) was used to detect HV antigen in rat lungs. Some M fragments in positive samples were amplified by reverse transcription-polymerase chain reaction (RT-PCR). Phylogenetic tree was constructed for phylogenetic analysis and typing . Results In 2008-2013, 1 838 catches were found in Jinzhou hemorrhagic fever epidemic area, belonging to 4 genera, 2 families and 5 species. The virus carrying rate was 4.24% (78/1 838) (Χ2 = 13.579, P = 0.019, P <0.05). The sequence analysis of some M fragments showed that the homology of the 11 strains of viruses with the Seoul virus (SEOV) nucleotide sequence was 96.2% -100% The homology of HTNV was only 70.6% ~ 71.2%. The phylogenetic tree showed that the 11 strains of SEO with same origin of the test had the same origin and the closest genetic relationship with Bj HD01 strain. Conclusion The predominant strains of HV in Jinzhou are SEO type, with small variation, relatively stable genotypes and obvious regional clustering.