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目的探讨ｎｍ２３－Ｈ１基因表达产物的表达水平与肺癌发生、发展和转移的关系。方法应用免疫组织化学法研究人非小细胞肺癌细胞中ｎｍ２３－Ｈ１基因表达产物的表达水平。结果ｎｍ２３－Ｈ１在肺癌组织中的表达水平（５４．０３％）明显低于癌旁正常肺组织（７３．４８％，Ｐ＜０．０１）。有淋巴结转移的原发癌ｎｍ２３－Ｈ１表达水平明显低于无淋巴结转移的原发癌（Ｐ＜０．０１）；转移癌组织表达水平明显低于原发癌组织（Ｐ＜０．０１）；低分化肺癌明显低于中－高分化肺癌（Ｐ＜０．０１）。结论ｎｍ２３－Ｈ１基因可能参与肺癌的发生、发展和转移过程的调控，ｎｍ２３－Ｈ１基因表达水平降低，预示肺癌的转移和预后不良。
Objective To investigate the relationship between the expression level of nm23-H1 gene and the occurrence, development and metastasis of lung cancer. Methods The expression of nm23-H1 gene expression product in human non-small cell lung cancer cells was studied by immunohistochemistry. Results The expression level of nm23-H1 in lung cancer tissues (54.03%) was significantly lower than that in normal lung tissues (73.48%, P<0.01). The expression of nm23-H1 in primary carcinoma with lymph node metastasis was significantly lower than that in primary carcinoma without lymph node metastasis (P<0.01); the expression level of metastatic carcinoma was significantly lower than that in primary carcinoma (P<0.01). Poorly differentiated lung cancer was significantly lower than moderately-differentiated lung cancer (P<0.01). Conclusion The nm23-H1 gene may be involved in the occurrence, development and metastasis of lung cancer. The expression level of nm23-H1 gene is decreased, indicating the metastasis and poor prognosis of lung cancer.