Metabolic-associated fatty liver disease(MAFLD)is the most common metabolic disease with a global prevalence of 25％.While MAFLD is serious and incurable at the later stage,it can be controlled or reversed at the early stage of hepatosteatosis originating from unhealthy diets.Recent laboratory evidence implicates a critical role of the mammalian target of rapamycin(mTOR)-autophagy signaling pathway in the pathogenesis of MAFLD induced by a high-fructose diet mimicking the overconsumption of sugar in humans.This review discusses the possible molecular mechanisms of mTOR-autophagy-endoplasmic reticulum(ER)stress in MAFLD.Based on careful analysis of recent studies,we suggest possible new therapeutic concepts or targets that can be explored for the discovery of new anti-MAFLD drugs.