以吉大豆1号、2号等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对不同基因型胚尖丛生芽诱芽率和再生率的影响,筛选得到适合于胚尖再生系统的基因型吉大豆2号,并以吉大豆2号为材料,研究侵染阶段加入脯氨酸和硝酸银对胚尖褐化率的影响,将获得的再生植株进行生根试验。结果表明:共培养基中添加3.0 mg.L-1脯氨酸和3.0 mg.L-1硝酸银能显著抑制褐化率,蔗糖配合较高浓度的IBA能更好地促进生根。 The effects of 6-BA with different concentrations on embryo bud budding rate and regeneration rate of different genotypes were studied by using 5 soybean varieties (Ji Soya No.1 and No.2) as materials and embryo tips as explants. In this study, the effects of proline and silver nitrate on the browning rate of the embryo were studied by using Ji Soya No.2 as the material, and the rooting experiment was conducted on the regenerated plants. The results showed that addition of 3.0 mg.L-1 proline and 3.0 mg.L-1 silver nitrate to the co-culture medium significantly inhibited the browning rate. Sucrose with higher concentration of IBA could better promote rooting.