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目的通过检测高糖和铁皮石斛多糖培养对高糖状态下大鼠视网膜Müller细胞活力和凋亡的调控,探讨铁皮石斛多糖保护糖尿病引起视网膜损伤的机制。方法大鼠视网膜Müller细胞用高糖(25 mmol/L)孵育48 h诱导损伤,同时用铁皮石斛多糖(100、200和400μg/ml)处理细胞48 h。实验分为正常对照组、铁皮石斛多糖(低、中、高剂量)组、高糖模型组、低、中、高剂量铁皮石斛多糖处理高糖组。采用MTT法和流式细胞术分别检测细胞活力和细胞凋亡,并计算细胞凋亡率。结果与对照组相比,高糖组Müller细胞活力显著降低(P<0.05),细胞凋亡率显著增加(P<0.05),铁皮石斛多糖(100、200和400μg/ml)没有显著影响Müller细胞活力和细胞凋亡(均P>0.05),与高糖组相比,高糖+铁皮石斛(100、200和400μg/ml)组Müller细胞活力显著增加(P<0.05),细胞凋亡率显著降低(P<0.05)。结论铁皮石斛多糖可能通过提高Müller细胞活性,减少Müller的凋亡,达到保护高糖状态下视网膜损伤的作用。
OBJECTIVE: To investigate the regulation of retinal Müller cell activity and apoptosis induced by high glucose and Dendrobium officinale in rats under high glucose conditions and to explore the mechanism of protective effects of Dendrobium candidum on diabetic retinopathy. Methods Rat retinal Müller cells were incubated with high glucose (25 mmol / L) for 48 h to induce injury, while cells were treated with dendrobium polysaccharide (100, 200 and 400 μg / ml) for 48 h. The experiment was divided into normal control group, Dendrobium candidum polysaccharide (low, medium and high dose) group, high glucose model group, low, medium and high doses of Dendrobium candidum polysaccharide treatment of high glucose group. MTT assay and flow cytometry were used to detect cell viability and apoptosis, and calculate the rate of apoptosis. Results Compared with the control group, the activity of Müller cells in high glucose group was significantly decreased (P <0.05) and the apoptosis rate was significantly increased (P <0.05). Dendrobium officinale polysaccharides (100, 200 and 400 μg / ml) did not significantly affect Müller cells (P <0.05). Compared with high glucose group, the activity of Müller cells in high glucose + Dendrobium officinale (100, 200 and 400μg / ml) group was significantly increased (P <0.05) Decreased (P <0.05). Conclusions Dendrobium candidum polysaccharides may play a role in protecting retinal damage under high glucose condition by increasing Müller cell activity and decreasing Müller apoptosis.