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本文报导了用负染法对１９９４年辽宁地区中国对虾大量死亡之病原病毒的检测结果，同时进行超薄切片证实。病虾病变组织匀浆上清液负染和病灶组织超薄切片电镜下可见一种长３５０～４００ｎｍ，直径８０～１００ｎｍ（切片中长２７０～３５０ｎｍ，直径１００～１２０ｎｍ），带囊膜的杆状病毒粒子。该病毒存在于病虾的胃肠组织，淋巴组织，皮下组织及肌肉组织中。该病毒粒子可见核衣壳的螺旋结构。该病毒引起中国对虾死亡的病原性已通过感染实验证实。作者认为负染法可做为对虾暴发性流行病的快速诊断方法。
This article reports the results of a negative staining method on the detection of a large number of pathogenic viruses that died in 1994 in the Liaoning area of China and confirmed by ultrathin sections. Negative staining of diseased tissue of diseased shrimp and negative staining of diseased tissue can be seen under the electron microscope with a length of 350-400 nm and a diameter of 80-100 nm (length of 270-350 nm and diameter of 100-120 nm) Viroid particles. The virus exists in diseased shrimp gastrointestinal tissue, lymphoid tissue, subcutaneous tissue and muscle tissue. The virus particles visible nucleocapsid spiral structure. The pathogenicity of the virus that caused the death of P. shrimp in China has been confirmed by infection experiments. The authors believe that negative staining can be used as a rapid diagnosis of fulminant shrimp epidemic.