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目的 研究肺鳞状细胞癌、腺癌、大细胞肺癌、小细胞肺癌以及淋巴结或肺内转移性肿瘤标本组织中p63基因的mRNA转录因子和蛋白表达水平,探讨p63基因表达与其定位在3q 27-q29区域改变的关系。方法 采用cDNA微阵列技术同时检测72例不同病理组织学类型的原发性肺癌(包括鳞状细胞癌、腺癌、大细胞癌、小细胞癌)和肺癌肺内转移及淋巴结转移灶内的p63基因mRNA水平。另外,利用组织芯片技术构建150例原发性肺癌石蜡包埋标本组织芯片,采用免疫组织化学LSAB法检测p63基因蛋白表达情况。同时应用比较基因组杂交(CGH)技术对70例原发性肺癌标本进行了3号染色体长臂改变的分析。结果 p63mRNA转录因子在肺鳞状细胞癌标本表达明显增强,与腺癌、大细胞癌、小细胞癌相比增多10倍以上。肺癌转移灶内p63基因mRNA表达水平明显高于其相对应的原发性灶,差异有统计学意义(P<0.001)。免疫组织化学染色结果显示:肺鳞状细胞癌阳性表达率为94.64％;腺癌是1.79％;4例大细胞肺癌中2例为阳性染色;但所有小细胞肺癌标本免疫组织化学染色均为阴性。pT1分期肺癌的p63基因蛋白表达阳性率与pT2分期肺癌相比有统计学差异(P<0.05)。比较基因组杂交结果发现:肺鳞状细胞癌3号染色体长臂27-29区域有显著的扩增,腺癌表现为缺失。鳞状细胞癌p63基因的?
Objective To study the mRNA and protein expression of p63 in lung squamous cell carcinoma, adenocarcinoma, large cell lung cancer, small cell lung cancer and lymph node or lung metastatic tumor tissue, and to investigate the relationship between p63 gene expression and its expression in 3q 27- q29 regional changes in the relationship. Methods 72 cases of primary lung cancer (including squamous cell carcinoma, adenocarcinoma, large cell carcinoma and small cell carcinoma) of different histopathological types and lung metastasis and lymph node metastasis of lung cancer were detected by cDNA microarray simultaneously. Gene mRNA levels. In addition, tissue microarray technique was used to construct tissue microarray specimens of 150 cases of primary lung cancer paraffin-embedded specimens and immunohistochemistry LSAB method was used to detect p63 gene protein expression. At the same time, comparative analysis of genetic hybridization (CGH) in 70 cases of primary lung cancer specimens were analyzed on chromosome 3 long arm changes. Results The expression of p63 mRNA was significantly increased in lung squamous cell carcinoma specimens, which was 10 times more than that in adenocarcinoma, large cell carcinoma and small cell carcinoma. The expression level of p63 mRNA in lung cancer metastasis was significantly higher than that of the corresponding primary tumor, the difference was statistically significant (P <0.001). Immunohistochemical staining results showed that the positive rate of squamous cell carcinoma was 94.64%, that of adenocarcinoma was 1.79%, and that of 4 cases of large cell lung cancer was positive, but all the small cell lung cancer samples were negative by immunohistochemical staining . The positive rate of p63 gene protein expression in pT1 stage lung cancer was significantly higher than that in pT2 stage lung cancer (P <0.05). Comparison of genomic hybridization results found: lung squamous cell carcinoma on chromosome 3 long arm 27-29 region has a significant amplification, the performance of adenocarcinoma is missing. Squamous cell carcinoma p63 gene?