应用二次正交旋转回归设计,研究培养基关键影响的四因子对大豆根腐生防菌AF-67发酵菌液浓度的影响.优化发酵培养基各成分的最佳配比为:牛肉浸膏1%,蛋白胨3.5%,可溶性淀粉1.8%,葡萄糖1%,发酵24h后稀释10倍工作液的实际光密度值A_(620)为0.743,较LB增殖培养液提高了42%.肯定了模型的可靠性和应用的有效性. The quadratic orthogonal rotation regression design was used to study the effect of the four key factors of culture medium on the concentration of Bacillus licheniformis AF-67 fermentation broth.The optimum proportion of each component of optimized fermentation medium was: beef extract 1 %, Peptone 3.5%, soluble starch 1.8%, glucose 1%, the actual optical density A_ (620) of 0.743, which was diluted 10 times after fermentation for 24 hours, was 42% higher than that of LB culture medium. The effectiveness of sexual and applied.