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目的 应用细胞培养技术检测了墓头回水提物对人脐血基质细胞生成及其分泌粒系造血刺激因子的实验研究。方法 将不同浓度的墓头回水提物与脐血共同培养 14d,检测基质细胞上清液中的 GM- CSF活性。结果 墓头回水提物对人脐血基质细胞具有促进形成的作用 ,最高峰为 0 .0 1μg/ m L,为了检测基质细胞上清液中的 GM-CSF活性 ,进行了 GM- CFU实验 ,结果表明 ,实验各组数据与对照组比较 ,具有非常显著性差异 (P<0 .0 1)。结论 墓头回水提物不仅是一种强烈的抗癌药物 ,也是一种造血祖细胞促进剂
Objective To detect the production of human umbilical cord blood stromal cells and the secretory granulocyte hematopoietic stimulating factor by the method of cell culture. Methods The umbilical cord blood was co-cultured with different concentrations of tocopherol extracts for 14 days, and the GM-CSF activity in stromal cell supernatant was detected. Results The water extract from the head of the toast had an effect on promoting the formation of human umbilical cord blood stromal cells, with the highest peak at 0.01 μg/m L. In order to detect the activity of GM-CSF in the stromal cell supernatant, a GM-CFU experiment was performed. The results showed that the experimental data of each group was significantly different from the control group (P<0.01). Conclusion The water extract from the head of the tomb is not only a strong anticancer drug but also a hematopoietic progenitor.