论文部分内容阅读
雄性SD大鼠60只,随机分成6组,二组对照,其余4组用昆明山海棠稀醇提取物(ATH)灌胃给药,每日分别为2.0g/kg;1.0g/kg;及0.3g/kg(二组),每周六次。用药6周至完全不育后剖杀用药的前三组及一组对照。取睾丸、附睾、心、肝、脾、肺、肾、胃,行常规HE及PAS+苏木素染色,部分鼠做DNA、RNA组化反应。取附睾尾评价精子数据。0.3g/kg组及另一组对照继续以每日0.2g/kg的维持量灌胃16周以保持不育,停药5周后证实其生育力恢复后剖杀,观察指标同前。结果表明:2.0g/kg组曲细精管平均损伤率为55.4%,1.0g/kg组曲细精管损伤明显少于前组(损伤率6.2％),小剂量鼠无论给药6周或22周睾丸和附睾形态与对照无异。结果提示:大鼠睾丸曲细精管的损伤范围和损伤的程度与ATH的剂量大小有关,而与作用时间的长短关系不大。最低抗生育剂量时,对ATH最敏感的部位是变态期精子细胞及附睾精子,以此剂量长期给药对睾丸附睾无明显影响,所致不育可以恢复。
Sixty male Sprague-Dawley rats were randomly divided into 6 groups and 2 groups. The other 4 groups were given gavage with ATH, 2.0g / kg and 1.0g / kg respectively; and 0.3g / kg (two groups), six times a week. After 6 weeks of treatment to complete infertility after the first three groups and a group of control drugs. Testis, epididymis, heart, liver, spleen, lung, kidney, stomach, routine HE and PAS + hematoxylin staining, some mice do DNA, RNA histochemical reaction. Epididymal tail evaluation sperm data. 0.3g / kg group and another group of control continue to 0.2g / kg daily maintenance dose of gavage for 16 weeks to maintain sterility, withdrawal 5 weeks after confirmation of fertility recovery after dissection, with the same indicators as before. The results showed that the average damage rate of the seminiferous tubules in the 2.0g / kg group was 55.4%. The damage of the seminiferous tubules in 1.0g / kg group was significantly less than that of the former group (6.2% injury rate) Testicular and epididymal morphology and no difference. The results suggest that the extent of damage and damage of testicular seminiferous tubules in rats is related to the dose of ATH but not to the duration of action. The lowest anti-fertility dose, the most sensitive parts of ATH is metamorphosis sperm cells and epididymal sperm, long-term administration of this dose of testicular epididymis had no significant effect, resulting in infertility can be restored.