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在36℃培育的小白鼠脑切片对~3H-GABA的摄取是浓度依赖性的、可饱和的,当2ml 培育液中注入100μl 浓度为215×10~(-4)mol/L 的~3H-GABA 时,脑片对 ~3H-GABA 的摄取达到饱和。3℃时切制脑片能获得较好的~3H-GABA 摄取效果。苄青霉素钾(PG)7.6×10~(-4)mol/L、苯巴比妥钠(PhB)9.8×10~(-4)mol/L、Gabaculline 4.0mol/L 各100μl 均使脑片对~3H-GABA 的摄取明显下降,荷包牡丹碱(BiC)0.25×10~(-4)mol/L、氨氧乙酸(AOAAA)1.0×10~(-4)mol/L 可使脑片对~3H-GABA 的摄取明显上升。
The uptake of 3H-GABA in brain slices incubated at 36 ℃ was concentration-dependent and saturable when 100μl of ~ 3H-GABA at a concentration of 215 × 10 ~ (-4) mol / At GABA, brain slices saturated with ~ 3H-GABA. 3 ℃ when cutting brain slices can get better ~ 3H-GABA uptake effect. Methyl gallate (7.6 × 10 -4 mol / L), phenobarbital sodium (9.8 × 10 -4 mol / L) and Gabaculline (4.0 mol / L) The uptake of 3H-GABA decreased obviously, and the dose of 0.25 × 10 ~ (-4) mol / L and 1.0 × 10 ~ (-4) mol / L of bicuculline (BiC) 3H-GABA uptake increased significantly.