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本文报导一种较简便而可同时检测不同淋巴细胞亚群的方法。兹简介于下: 1.用Ficoll-Hypaque分离淋巴细胞,污染的红细胞用氯化铵Tris缓冲液溶解,淋巴细胞再用磷酸缓冲盐水(PBS)洗涤三次,调整细胞数到5×10~8/毫升。
This article reports a simpler method for simultaneous detection of different lymphocyte subsets. Here is a brief description of the following: 1. Lymphocytes were isolated using Ficoll-Hypaque, and the contaminated erythrocytes were dissolved in Tris-HCl buffer. Lymphocytes were washed three times with phosphate-buffered saline (PBS) and adjusted to 5x10-8 / Ml.