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目的 :建立一种脑细胞缺血离体模型。方法 :大鼠脑突触体分别在不同条件下培养 ,电镜观察形态结构 ,测定其生物活性。结果 :突触体结构正常 ,内含线粒体与递质囊泡 ,缺血培养对形态结构无明显影响。突触体悬液与上清液LDH活性的比值在正常和缺血培养条件没有明显差异 ,而缺血培养可使突触体内游离钙浓度显著升高 (P <0 0 5) ,高钾刺激可使其进一步增高 (P <0 0 1 )。结论 :大鼠脑突触体经体外缺血培养后 ,仍保持完整的形态结构和良好生物活性
Objective: To establish a model of cerebral ischemia in vitro. Methods: Rat brain synaptosomes were cultured under different conditions. Morphology and structure were observed under electron microscope. Results: The structure of synaptosome was normal, containing mitochondria and neurotransmitter vesicles. The ischemic culture had no significant effect on the morphology and structure. The ratio of LDH activity in synaptosome suspension and supernatant did not differ significantly between normal and ischemic culture conditions, but ischemic culture could significantly increase the concentration of free calcium in synaptosomes (P <0 05) Can make it further increased (P <0 0 1). CONCLUSIONS: Rat cerebral synaptosomes remain intact morphological structure and good biological activity after being cultured in vitro