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目的:评价小鼠许旺细胞体外复合改性聚乳酸聚羟基乙酸(PLAPGA)的细胞活性及生物相容性。方法:转绿色荧光蛋白基因(GFP)小鼠的许旺细胞传代培养至第2代,然后通过MTT检测在不同改性技术(H_2O_2、NaOH、NaClO_4、K_2CrO_4及超声波)处理的PLAPGA浸提液中许旺细胞的增殖情况,检测许旺细胞在PLAPGA表面的黏附及其细胞形态。结果:于培养1d,3d测得在不同改性技术处理的PLAPGA浸提液OD值,1天时,各浸提液组和对照组相比无显著性差异,许旺细胞的活力及增殖无影响。3天时,经NaClO_4及K_2CrO_4处理的PLAPGA与对照组相比具有统计学差异,影响许旺细胞的增殖,对许旺细胞有毒性;荧光显微镜下观察到许旺细胞在改性PLAPGA表面逐渐伸展,形成伪足,最终粘附在材料表面。结论:经H_2O_2、NaOH及超声波改性PLAPGA无细胞毒性,具有良好的生物相容性和黏附性,可以用于组织工程化神经的构筑。
OBJECTIVE: To evaluate the cell viability and biocompatibility of mouse Schwann cells in vitro with modified polylactic acid polyglycolic acid (PLA PGA). Methods: Schwann cells transfected with green fluorescent protein (GFP) mice were subcultured into passage 2 and then treated with different modified techniques (H 2 O 2, NaOH, NaClO 4, K 2 CrO 4 and ultrasound) by MTT Proliferation of Schwann cells in the extract, the detection of Schwann cells in PLA PGA surface adhesion and cell morphology. Results: The OD values of PLA PGA extracts treated with different modified technologies at 1 day and 3 days of culture showed no significant difference compared with the control group on the day 1, and the activity of Schwann cells and No effect on proliferation. At 3 days, PLA PGA treated with NaClO_4 and K_2CrO_4 showed statistical difference compared with the control group, which affected the proliferation of Schwann cells and the toxicity of Schwann cells. The results showed that the Schwann cells in modified PLA PGA surface gradually extended to form pseudo-foot, the final adhesion to the surface of the material. CONCLUSION: The cytotoxicity of PLA PGA modified by H2O2, NaOH and ultrasound is good and has good biocompatibility and adhesion. It can be used in the construction of tissue engineered nerves.