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目的探讨焦炉工GSTM1和GSTT1基因多态性与多环芳烃类(PAHs)代谢为1-羟基芘(1-OHP)过程之间的关系。方法以某焦化厂焦炉车间生产工人150人和其他车间无职业性PAHs接触者39人为调查对象。用高效液相色谱法测定尿中1-OHP浓度;聚合酶链反应一限制性片段长度多态性(PCRRFLP)法分别检测GSTM1和GSTT1基因多态性。采用方差分析和多因素回归分析等统计学方法研究影响尿中1-OHP浓度的因素。结果尿中1-OHP浓度与焦炉工作业的位置呈正相关(P<0.01)。低暴露组GSTT1(阴性)个体尿中1-OHP浓度较GSTT1(阳性)个体高;GSTM1(阴性)/GSTT1(阴性)全缺失型个体尿中1-OHP浓度明显高于GSTM1(阳性)/GSTT1(阳性)全野生型个体(P<0.05)。结论在PAHs低暴露水平下,GSTT1可以影响PAHs代谢。GSTM1和GSTT1基因的交互作用可以影响焦炉工PAHs代谢为1-OHP的过程。
Objective To investigate the relationship between GSTM1 and GSTT1 gene polymorphism and the metabolism of 1-hydroxypyrene (1-OHP) in PAHs. Methods A total of 150 workers in a coke oven plant and 39 workers without occupational PAHs in other workshops were investigated. The concentration of 1-OHP in urine was determined by HPLC. The polymorphisms of GSTM1 and GSTT1 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP). Analysis of variance and multivariate regression analysis and other statistical methods to study the impact of urinary 1-OHP concentration factors. Results The urinary 1-OHP concentration was positively correlated with the location of coke oven workers (P <0.01). Urinary 1-OHP concentrations in individuals with GSTT1 (negative) were significantly higher than those with GSTT1 (positive) in low exposure groups; urinary 1-OHP concentrations were significantly higher in GSTM1 (negative) / GSTT1 (negative) individuals than those with GSTM1 (positive) / GSTT1 (Positive) whole-wild-type individuals (P <0.05). Conclusions GSTT1 can affect the metabolism of PAHs under the low level of PAHs exposure. The interaction of GSTM1 and GSTT1 genes can affect the metabolism of coke oven workers PAHs to 1-OHP.