论文部分内容阅读
目的探讨HMGA1通过Wnt/β-Catenin通路在胃肿瘤形成中的作用。方法应用小干扰RNA介导的基因沉默、细胞增殖分析、PCR等技术完成实验。结果 HMGA1的特异敲除明显减少细胞生长。β-Catenin或下游c-myc的损失减少HMGA1表达,而Wnt3a处理增加HMGA1和c-myc的转录。结论这些数据表明,HMGA1参与胃癌细胞形成和增殖通过Wnt/β-Catenin通路。
Objective To investigate the role of HMGA1 in gastric neoplasia through Wnt / β-Catenin pathway. Methods Small RNA interference mediated gene silencing, cell proliferation assay, PCR and other techniques to complete the experiment. Results Specific knockout of HMGA1 significantly reduced cell growth. Loss of β-Catenin or downstream c-myc decreases HMGA1 expression, whereas Wnt3a treatment increases transcription of HMGA1 and c-myc. Conclusions These data suggest that HMGA1 is involved in gastric cancer cell formation and proliferation through the Wnt / β-Catenin pathway.