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目的:构建介导KCNQ2野生型及其突变的真核表达载体,研究其在细胞中的定位及其质膜表面的表达,并利用全细胞膜片钳技术观察电流特性,探讨KCNQ2通道在良性家族性新生儿惊厥(BFNC)中的发病机制。方法:采用PCR方法扩增目的基因,分别构建野生型pEGFP-C1-KCNQ2和突变型pEGFP-C1-KC-NQ
OBJECTIVE: To construct eukaryotic expression vector that mediates wild type and its mutation of KCNQ2, to study its localization in the cell and the expression of its plasma membrane surface. Whole cell patch clamp technique was used to observe the current characteristics and explore the role of KCNQ2 channel in benign familial Neonatal convulsions (BFNC) in the pathogenesis. Methods: The target gene was amplified by PCR and the wild-type pEGFP-C1-KCNQ2 and mutant pEGFP-C1-KC-NQ