Objective:To investigate the expression of hENTl andERCC1 genes in tumor tissues non-small cell lung cancer(NSCLC).Methods:Fresh non-small lung cancer specimens were transplanted into nude mice.Twenty mice were randomized into two groups: experimental group receiving gemcitabine plus cisplatin and control group receiving0.9% physiological saline.The expressions of hENTl andERCC1 mRNA in tumor tissue were detected by real-time fluorescent quantitative PCR.The volume of tumor, the weight of nude mice and tumor volume were respectively measured and calculated2-3 times per week.Tissue samples were collected fromNSCLC mice treated with gemcitabine plus carboplatin.Results:The histological examination showed that many tumor cells were well preserved in nude mice.The rate of transplanted tumor cells was 86.7%.The concomitant treatment study showed that the rate ofTV,RTV,T/C inGEM+DDP group was the lowest.LBP+DOC,DDP+DOC obviously influenced the body weight.Compared with NS group,DDP group,GEM group, the survival period and the level of hENTl ofDDP+GEM group increased obviously, the level ofERCC1 decreased significantly(P<0.05).Conclusions:The expression of hENT1 andERCC1 genes in tumor tissues were closely correlated with the response to chemotherapy and prognosis of patients withNSCLC treated with gemcitabine plus cisplatin.