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目的 :探讨孕早期母胎免疫耐受调节机制。方法 :应用流式细胞仪分析 (FACS)蜕膜及孕妇外周血淋巴细胞亚群 ;采用改进的乳酸脱氢酶释放试验测定蜕膜淋巴细胞对K5 6 2 的自然杀伤活性 ;同时以滋养细胞与蜕膜淋巴细胞共同培养 ,研究母体免疫系统对滋养细胞抗原免疫应答状态。结果 :蜕膜组织中CD5 6 + NK细胞含量高于孕妇及对照组外周血CD5 6 + 细胞比例 (P <0 .0 5 ) ;蜕膜组织中NK细胞杀伤活性明显低于孕妇及对照组外周血NK细胞杀伤活性 (P <0 .0 5 ) ;滋养细胞抑制母方淋巴细胞增殖反应 (P <0 .0 5 )。结论 :滋养细胞调低蜕膜淋巴细胞增殖反应 ,蜕膜CD5 6 + NK细胞自然杀伤活性受抑制 ,可能是维持正常妊娠的重要机制
Objective: To explore the regulation mechanism of immune tolerance in the first trimester of pregnancy. Methods: The peripheral blood lymphocyte subpopulations of decidua and pregnant women were analyzed by flow cytometry (FCM). The natural killer activity of decidual lymphocytes on K562 cells was determined by modified lactate dehydrogenase release assay. At the same time, Decidual lymphocytes co-cultured to study the immune system of the maternal immune response to trophoblastic antigen status. Results: The content of CD56 + NK cells in decidua tissue was higher than that in pregnant women and control group (P <0.05). The cytotoxic activity of NK cells in decidua was significantly lower than that in pregnant women and controls Blood NK cell killing activity (P <0.05); trophoblast cells inhibited the proliferation of maternal lymphocytes (P <0.05). CONCLUSION: Trophoblastic cells reduce the proliferative response of decidual lymphocytes, and the natural killer activity of decidual CD5 6 + NK cells is inhibited, which may be an important mechanism to maintain normal pregnancy