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目的观察氯化锂—匹罗卡品致皮质发育不良(disorders of cortical development,DCDs)大鼠癫痫持续状态(Status epylepticus SE)后行为学和脑电变化特点,探讨皮质发育不良与认知功能和癫痫的关系。方法建立卡莫司汀(BUCN)致皮质发育不良大鼠模型,用氯化锂—匹罗卡品诱导模型鼠SE发作,观察大鼠SE发作前后旷场活动、拒俘反应和水迷宫训练和脑电变化。结果(1)皮质发育不良组(DCDs组)大鼠旷场活动性减少,拒俘反应性增强,水迷宫训练潜伏期延长,与正常对照组(NC组)比较差异有显著性意义(P<0.05);两组大鼠脑电波均以α、β为主,未见痫性波发放。(2)两组大鼠致痫后认知功能较致痫前均显著降低(P<0.01-0.05),DCDs组降低较NC组更明显(P<0.01-0.05);DCDs组大鼠痫性发作潜伏期缩短,SE持续时间延长,与NC组比较差异有显著性意义(P<0.05);DCDs组大鼠SE后自发性痫性放电较NC组明显增多,波幅增加,组间比较差异有显著性意义(P<0.01-0.05)。结论DCDs可致大鼠认知功能下降、痫性发作潜伏期缩短和SE时间延长;DCDs大鼠SE后自发性痫性放电明显增强,进一步加重了认知功能的损害。
Objective To observe the changes of behavior and EEG after Status epilepticus SE in rats with disorders of cortical development (DCDs) induced by lithium chloride-pilocarpine to explore the relationship between cortical dysplasia and cognitive function and Relationship of epilepsy. Methods The model of cortical hypoplasia induced by Carmustine (BUCN) was established. SE was induced by lithium chloride-pilocarpine and the open field activity, captive reaction and water maze training were observed before and after the onset of SE EEG changes. Results (1) The cortical dysplasia group (DCDs group) decreased activity in the open field, refuse retentivity, water maze training latency prolonged, compared with the normal control group (NC group), there was significant difference (P <0.05 ); Both groups of brain electrical wave are α, β-based, no epileptic wave release. (2) Compared with preeclampsia, the cognitive function of the two groups was significantly lower than that of the preeclampsia group (P <0.01-0.05), the DCDs group was more obvious than the NC group (P <0.01-0.05) Compared with NC group, the latent period of SE was shortened and the duration of SE prolonged, which was significantly different from that of NC group (P <0.05). The spontaneous seizure discharge of SE rats in DCDs group was significantly higher than that in NC group Sexual significance (P <0.01-0.05). Conclusions DCDs can decrease the cognitive function, shorten the latent period of epileptic seizure and extend the duration of SE in seizures. The spontaneous seizure discharge of SE rats with DCDs is significantly increased, further aggravating the impairment of cognitive function.