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目的:研究美洲远志(Polygala senega)根的乙醇提取物对苯并芘致肺癌小鼠的抗肿瘤作用。方法:瑞士白化小鼠被分为5组,每组6只。组1为对照组,予口服橄榄油;组2予苯并芘(50mg/kg体质量,溶于橄榄油中),每周2次,连续4周;组3除予与组2相同的苯并芘外,再予48%乙醇;组4予与组2相同的苯并芘,并同时给予美洲远志的乙醇提取物,每日1次,连续16周;组5仅给予美洲远志的乙醇提取物,每日1次,连续16周。16周后,处死所有小鼠并检测和评价以下指标:2,2-二苯基-1-苦基肼(2,2-diphenyl-1-picrylhydrazyl,DPPH)法测定美洲远志乙醇提取物的抗氧化功效;分别称取各组小鼠的肺组织质量及体质量;彗星实验及酶联免疫吸附法检测DNA损伤情况;分别测定过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、脂质过氧化反应及总含硫化合物含量。结果:给予美洲远志乙醇提取物治疗的组4小鼠与组3模型组小鼠相比,体质量明显增加而肺组织质量明显降低(P<0.01)。彗星实验结果显示组4小鼠的DNA损伤较组3小鼠明显减轻(P<0.01)。酶联免疫吸附法测定p53蛋白水平,组4明显高于组3(P<0.01)。组2和组3的模型小鼠与组1比较,脂质过氧化反应水平明显升高,而抗氧化标志物的水平明显降低(P<0.05),组4小鼠的这些指标被明显逆转(P<0.01)。结论:美洲远志对于化学制剂致小鼠肺癌有明确的治疗效果。
Objective: To study the antitumor effect of ethanol extract of Polygala senega root on mice induced by benzopyrene. Methods: Swiss albino mice were divided into 5 groups with 6 mice in each group. Group 1 was given orally with olive oil; Group 2 was given benzopyrene (50 mg / kg body weight dissolved in olive oil) twice a week for 4 weeks; Group 3 was given the same benzene as group 2 And pyrene, and then to 48% ethanol; group 4 to the same group 2 benzo [a] pyrene, and at the same time given Polygala Polygalaceae ethanol extract once a day for 16 consecutive weeks; group 5 was given Polygala only ethanol extraction Objects, 1 day, for 16 weeks. After 16 weeks, all mice were sacrificed and the following indicators were tested and evaluated: 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for the detection of Polygala tenuifolia extract The lung tissue quality and body weight of each group were weighed. The DNA damage was detected by comet assay and enzyme-linked immunosorbent assay. The activities of catalase, superoxide dismutase, glutathione peroxidase Enzyme, glutathione reductase, lipid peroxidation and total sulfur compounds content. Results: Compared with mice in group 3, the mice in group 4 treated with ethanol extract of Polygala tenuifolia Will have significantly increased body weight and lung tissue quality (P <0.01). The results of comet assay showed that DNA damage in group 4 mice was significantly reduced compared with group 3 mice (P <0.01). The level of p53 protein was detected by enzyme-linked immunosorbent assay (ELISA), group 4 was significantly higher than group 3 (P <0.01). Compared with group 1, the level of lipid peroxidation and the levels of antioxidant markers in group 2 and group 3 were significantly decreased (P <0.05), and those in group 4 were significantly reversed ( P <0.01). Conclusion: Polygala Polygalae has a clear therapeutic effect on lung cancer caused by chemical agents in mice.