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利用露天盆栽试验研究了不同浓度Cd胁迫下转PprⅠ基因油菜和野生型油菜对Cd的吸收特性以及生理响应.结果表明:随着Cd胁迫浓度的升高,转基因和野生型油菜对Cd的吸收均显著增加,且二者地下部分的吸收都大于地上部分;在0.5mg/kg Cd处理下,转基因和非转基因油菜对Cd的吸收差异不显著,但1和2mg/kg处理下转基因油菜对Cd的吸收显著低于非转基因油菜.两种油菜对Cd的富集系数和转运系数均随处理浓度的升高而降低;随着Cd浓度的增加,超氧化物歧化酶(SOD)活性呈现先上升后下降的趋势,且在相同处理浓度下,转基因油菜的SOD活性均高于非转基因油菜;丙二醛(MDA)含量呈上升趋势,且其在转基因油菜中的含量均低于非转基因油菜;叶绿素a和叶绿素b的含量随Cd浓度的升高而下降.因此PprⅠ基因的导入,显著降低了油菜对Cd的吸收,提高了其可食部分安全性.
The pot experiments were conducted to study the Cd uptake and physiological response of PprⅠgene rape and wild-type rapeseed (Brassica napus L.) under Cd stress. The results showed that Cd uptake by transgenic and wild type rape (P <0.05). The uptake of Cd by both transgenic and non-transgenic rapeseeds was not significantly different at 0.5 mg / kg Cd treatment, but at 1 mg / kg and 2 mg / kg, The absorption of Cd was significantly lower than that of non-transgenic rape.The accumulation coefficient and translocation coefficient of Cd in both rapeseed decreased with the increase of Cd concentration.The superoxide dismutase (SOD) activity increased first and then increased with Cd concentration The SOD activity of transgenic rapeseed was higher than non-transgenic rapeseed at the same treatment concentration. The content of malondialdehyde (MDA) in the transgenic rapeseed increased and the content of MDA in the transgenic rapeseed was lower than that of the non-transgenic rape. a and chlorophyll b decreased with the increase of Cd concentration.The introduction of PprⅠ gene significantly reduced the Cd uptake by rapeseed and increased the edible part safety.