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本研究分别采用１２５Ⅰ标记的牛血清白蛋白测定肺微血管通透性；电子自旋共振仪测定组织中氧自由基（ＯＦＲ）含量；生化方法测定组织中磷脂酶Ａ２（ＰＬＡ２）活力、肝细胞活力和肠粘膜中二胺氧化酶含量。结果显示：急性重症胆管炎（ＡＣＳＴ）时大鼠肺脏、肝脏和肠粘膜功能受到严重损害，表现在肺微血管通透性与对照组相比明显增加（Ｐ＜０．０１），肝细胞活力和肠粘膜二胺氧化酶活力明显低于对照组（Ｐ＜０．０１），与此相平行，三种组织中的ＯＦＲ和ＰＬＡ２水平明显高于对照组（Ｐ＜０．０１）。中药组的治疗效果最佳，而减压组和氨苄组的治疗效果较差甚至加重某些组织的功能损伤。不同治疗方法的结果与其对不同组织中ＯＦＲ和ＰＬＡ２的影响密切相关。我们还发现在ＡＣＳＴ，不同组织中ＯＦＲ与ＰＬＡ２的水平并不相同，肝脏的ＯＦＲ及肠管的ＰＬＡ２最高，肺脏和肝脏中的ＯＦＲ高于外周血中的ＯＦＲ。提示在ＡＣＳＴ时ＯＦＲ和ＰＬＡ２的产生主要发生在器官水平，单纯测定血浆中的含量难以反映各个器官内的真实水平。在文中我们还对ＡＣＳＴ时组织损伤发生的病生理机制和中药的作用机理进行了探讨。
In this study, 125I-labeled bovine serum albumin was used to measure the permeability of the pulmonary microvasculature; the electron spin resonance spectrometer was used to determine the content of oxygen free radicals (OFR); the biochemical method was used to determine the phospholipase A2 (PLA2) activity and the liver cell viability in the tissue. Diamine oxidase content in the intestinal mucosa. The results showed that the lung, liver and intestinal mucosal functions of rats were severely impaired during acute severe cholangitis (ACST). The microvascular permeability in the lungs was significantly increased compared with the control group (P<0.01). The diamine oxidase activity in intestinal mucosa was significantly lower than that in the control group (P<0.01). Parallel to this, the OFR and PLA2 levels in the three tissues were significantly higher than those in the control group (P<0.01). The Chinese medicine group had the best therapeutic effect, while the decompression group and the ampicillin group had poor treatment effects and even aggravated the functional impairment of some tissues. The results of different treatment methods are closely related to their influence on OFR and PLA2 in different tissues. We also found that in ACST, the levels of OFR and PLA2 in different tissues are not the same, the OFR of the liver and the PLA2 of the intestine are the highest, and the OFR in the lung and liver is higher than the OFR in the peripheral blood. It is suggested that the production of OFR and PLA2 mainly occurs at the organ level at the time of ACST, and it is difficult to simply measure the plasma level to reflect the true level in each organ. In the article, we also discuss the physiological and pathological mechanisms of tissue damage and the mechanism of action of traditional Chinese medicine at the time of ACST.