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目的:探讨益肾降浊冲剂对阿霉素肾病大鼠肾小球硬化的干预作用与机制。方法:SD大鼠随机分为对照组、模型组、他汀组、益肾降浊冲剂(低、中、高剂量)组,每组6只。采用单侧肾切除加阿霉素重复注射建立肾小球硬化模型。用药8周后观察各组大鼠24h尿蛋白定量、血生化、氧化低密度脂蛋白(ox-LDL)的变化,PAS、Masson染色评估肾小球硬化程度,免疫组化观察肾小球氧化低密度脂蛋白受体(LOX-1)的表达,Western Blot检测Ⅰ型胶原(ColⅠ)、纤连蛋白(FN)的表达。结果:与对照组比较,模型组大鼠24h尿蛋白量、尿素氮(BUN)、肌酐(Scr)、胆固醇(Cho)、低密度脂蛋白胆固醇(LDL-C)、ox-LDL、肾小球硬化评分、系膜肾小球系膜基质比(M/G)均明显升高,肾小球LOX-1、ColⅠ、FN表达显著增加(P<0.01)。与模型组比较,益肾降浊冲剂组呈剂量依赖性降低24h尿蛋白量、Bun、Scr、Cho、LDL-C、ox-LDL水平以及肾小球硬化评分、M/G(P<0.01),下调LOX-1、ColⅠ、FN的表达(P<0.01)。结论:益肾降浊冲剂组能通过纠正脂代谢紊乱、抑制LOX-1的表达、减轻细胞外基质的沉积、改善肾小球硬化。
Objective: To investigate the effect and mechanism of Yishen Jiangzhuo granule on glomerulosclerosis in adriamycin-induced nephropathy rats. Methods: SD rats were randomly divided into control group, model group, statin group and Yishen Jiangzhu granules (low, medium and high dose) group, with 6 rats in each group. The model of glomerulosclerosis was established by repeated injection of unilateral nephrectomy plus doxorubicin. After 8 weeks of treatment, 24-hour urinary protein excretion, blood biochemical and oxidized low density lipoprotein (ox-LDL) were observed and the degree of glomerulosclerosis was assessed by PAS and Masson staining. The glomerular oxidation was observed by immunohistochemistry The expression of LOX-1 was detected by Western Blot. The expressions of collagen Ⅰ (ColⅠ) and fibronectin (FN) were detected by Western Blot. Results: Compared with the control group, 24h urinary protein, BUN, Scr, LD, LDL-C, ox-LDL, Sclerotic score, mesangial matrix ratio (M / G) of glomerular mesangium were significantly increased, and the expression of LOX-1, ColⅠ, FN in glomerulus was significantly increased (P <0.01). Compared with the model group, the Yishen Jiangzhuo Granule group reduced the levels of urinary protein, the levels of Bun, Scr, Cho, LDL-C, ox-LDL and the score of glomerular sclerosis, M / G in a dose- , Down-regulated the expression of LOX-1, ColⅠ, FN (P <0.01). Conclusion: Yishen Jiangzhu granule can correct glioma disorder, inhibit the expression of LOX-1, reduce the deposition of extracellular matrix and improve glomerulosclerosis.