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目的研究卵巢原发癌和转移癌组织中Survivin mRNA、Ki67蛋白表达的相关关系及临床意义。方法对1985~2002年中国医科大学附属一院妇科收治的38例卵巢原发癌、36例卵巢转移癌、16例卵巢良性肿瘤病例组织标本及10例正常卵巢组织运用原位杂交技术检测Survivin mRNA的表达,免疫组化SP法检测Ki67蛋白标记指数,并对其与临床各因素关系进行综合分析。结果Survivin、Ki67在卵巢原发癌和转移癌组织中的表达水平明显高于正常卵巢及良性卵巢肿瘤组织(P<0·01)。Ki67表达与年龄及有无淋巴结转移无关,与卵巢原发癌和转移癌的临床分期、分化程度及卵巢转移癌的预后密切相关。Survivin表达与Ki67表达具有一定关联性,呈正相关关系(r=0·498)。结论Survivin和Ki67的高表达在卵巢原发癌及转移癌的发生发展中有协同作用,可作为评价卵巢肿瘤生物学行为的重要依据,并可评估卵巢肿瘤恶性程度的重要参考指标。
Objective To study the correlation between the expression of Survivin mRNA and Ki67 protein in primary ovarian cancer and metastatic carcinoma and its clinical significance. Methods 38 cases of primary ovarian cancer, 36 cases of ovarian metastasis, 16 cases of ovarian benign tumor and 10 cases of normal ovarian tissue were enrolled in the First Affiliated Hospital of China Medical University from 1985 to 2002. Survivin mRNA was detected by in situ hybridization The expression of Ki67 protein index was detected by immunohistochemical SP method and the relationship between Ki67 protein index and clinical factors was analyzed. Results The expression of Survivin and Ki67 in primary ovarian cancer and metastatic carcinoma tissues was significantly higher than that in normal ovarian tissues and benign ovarian tumor tissues (P <0.01). Ki67 expression and age and lymph node metastasis has nothing to do with the primary ovarian cancer and metastasis of clinical stage, the degree of differentiation and the prognosis of metastatic ovarian cancer are closely related. There was a positive correlation between Survivin expression and Ki67 expression (r = 0.498). Conclusion The overexpression of Survivin and Ki67 has a synergistic effect in the development and progression of primary ovarian cancer and metastatic carcinoma. It may serve as an important basis for evaluating the biological behavior of ovarian tumors and may be an important reference index for assessing the malignancy of ovarian tumors.