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在以正己烷-异丙醇为移动相的体系中,用Chiralcel OD,Chiralcel OJ及Chiramalpak AD作为手性固定相对13种β-氨基醇及β-羟基硫醚类化合物对映体进行HPLC手性拆分,这些化合物至少能在一支柱上得到基线级分离。考察了它们于不同浓度配比的这类洗脱体系中在柱上的色谱行为。实验表明化合物取代基的性质明显影响它们在手性柱上的拆分。手性固定相与外消旋样品上的极性基因之间的氢键作用和π-π作用可能是进行手性识别的主要原因。方法已用于非手性环氧化合物不对称开环反应产物β-氨基醇及β-羟基硫醚类化合物的光学纯度鉴定。
The enantiomers of 13 β-amino alcohols and β-hydroxythioethers were separated by chiral HPLC using Chiralcel OD, Chiralcel OJ and Chiramalpak AD as chiral stationary phases in a mobile phase with n-hexane-isopropanol as mobile phase Resolution, these compounds at least in a column to get baseline-level separation. Their chromatographic behavior on the column in this type of elution system with different concentration ratios was investigated. Experiments show that the nature of the substituents of the compounds significantly affects their resolution on chiral columns. Hydrogen bonding and π-π interaction between the polar genes on the chiral stationary phase and the racemic sample may be the main reason for chiral recognition. The method has been used for the optical purity identification of asymmetric ring-opening reaction products of β-amino alcohols and β-hydroxythioethers of achiral epoxy compounds.