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目的:探讨尿激酶型纤溶酶原激活剂(u-PA)、其受体(u-PAR)和其抑制剂-1(PAI-1)在PCOS发病机制中的作用。方法:采用睾酮或孕酮联合hCG刺激建立PCOS大鼠模型;免疫组化S-P法测定u-PA、u-PAR和PAI-1在模型动物卵巢组织中的分布与表达。结果:u-PA、u-PAR在PCOS组颗粒细胞和泡膜细胞中的染色比正常对照组明显减弱(P<0.05),PAI-1在泡膜细胞及间质细胞中的染色均比正常对照组明显增强(P<0.05)。结论:u-PA、u-PAR及PAI-1的表达异常可能参与PCOS的发病。
Objective: To investigate the role of urokinase-type plasminogen activator (u-PA), its receptor (u-PAR) and its inhibitor-1 (PAI-1) in the pathogenesis of PCOS. Methods: PCOS rat model was established by testosterone or progesterone combined with hCG stimulation. The distribution and expression of u-PA, u-PAR and PAI-1 in ovarian tissue of model animals were detected by immunohistochemical S-P method. Results: The staining of u-PA and u-PAR in PCOS group was significantly weaker than that of the normal control group (P <0.05). The staining of PAI-1 in the foam cells and stromal cells was more than normal The control group was significantly enhanced (P <0.05). Conclusion: The abnormal expression of u-PA, u-PAR and PAI-1 may be involved in the pathogenesis of PCOS.