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目的探讨肿瘤坏死因子诱导凋亡配体(TRAIL)对胃癌细胞株MKN28细胞体外诱导凋亡的作用及机制。方法采用MTT法培养、分析细胞增殖抑制率及细胞存活率,流式细胞仪分析细胞凋亡及细胞周期,AO/EB染色观察凋亡细胞。结果 TRAIL可明显抑制MKN28细胞的增殖,并可诱导细胞的凋亡。结论 TRAIL可诱导MKN28细胞株的凋亡,并呈剂量依赖性。
Objective To investigate the effect and mechanism of tumor necrosis factor-induced apoptosis ligand (TRAIL) on apoptosis of gastric cancer cell line MKN28 in vitro. Methods MTT method was used to culture and analyze cell proliferation inhibition rate and cell survival rate. Flow cytometry was used to analyze apoptosis and cell cycle. AO / EB staining was used to observe apoptotic cells. Results TRAIL significantly inhibited the proliferation of MKN28 cells and induced cell apoptosis. Conclusion TRAIL can induce the apoptosis of MKN28 cells in a dose-dependent manner.