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用多聚酶链反应检测子宫颈癌组织HPV16E6、E7基因,同时用基因工程方法制备E6、E7抗原,以免疫酶联吸附法检测其血清抗体。结果表明,宫颈癌组织中HPV16E6、E7DNA检出总阳性率为71.8%,E6、E7基因检出率分别为37.5%(12/32)和65.6%(21/32)。HPV16E6、E7血清抗体反应
The HPV16E6 and E7 genes were detected by polymerase chain reaction (PCR) and E6 and E7 antigens were detected by genetic engineering. The serum antibodies were detected by enzyme-linked immunosorbent assay. The results showed that the positive rates of HPV16E6 and E7DNA in cervical cancer were 71.8% and 37.5% (12/32) and 65.6% (21/32), respectively. HPV16E6, E7 serum antibody response