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目的探讨肝X受体(liver X receptors,LXRs)的特异性激动剂GW3965在Raji细胞中对B淋巴细胞刺激因子(B lymphocyte stimulator,BLyS)表达的影响。方法 MTT法检测不同浓度的GW3965(浓度分别为0.5、5、10μmol/L)对Raji细胞活性的影响,观察时间为加入GW3965共孵育后24 h。用LXR的特异性激动剂GW3965刺激人B细胞淋巴瘤株Raji细胞,经RT-PCR检测LXR特异性靶基因三磷酸腺苷结合盒A1(ATP-binding cassette,ABCA1)mRNA的表达;经RT-PCR和Western blot检测BLyS的表达。结果对照组1‰DMSO D(492)为(0.632±0.055),0.5、5μmol/L和10μmol/L的GW3965 D(492)为(0.609±0.073)、(0.612±0.052)和(0.628±0.055)。LXR的特异性配体GW3965对细胞活性无影响(P>0.05)。GW3965作用于Raji细胞后,ABCA1 mRNA表达剂量依赖性上调,LXR活化后可在转录和翻译水平剂量依赖性下调抑制BLyS的表达。结论 LXR在Raji细胞中可抑制BLyS的表达。
Objective To investigate the effect of GW3965, a specific agonist of liver X receptors (LXRs), on the expression of B lymphocyte stimulator (BLyS) in Raji cells. Methods The effects of different concentrations of GW3965 (concentration of 0.5, 5, 10μmol / L) on the activity of Raji cells were detected by MTT assay. The observation time was 24 h after co-incubation with GW3965. The human B cell lymphoma Raji cells were stimulated with LXR-specific agonist GW3965 and the expression of LXR-specific target gene ATP-binding cassette (ABCA1) mRNA was detected by RT-PCR. blot detection BLyS expression. The results showed that the control group showed a significant increase in GW3965 D (492), (0.660 ± 0.052) and (0.628 ± 0.055) . GW3965, a specific ligand of LXR, had no effect on cell viability (P> 0.05). After treated with GW3965, the expression of ABCA1 mRNA was up-regulated in a dose-dependent manner. LXR inhibited the expression of BLyS in a dose-dependent and down-regulated manner at transcriptional and translational level. Conclusion LXR can inhibit the expression of BLyS in Raji cells.