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目的本实验以１０ｕｓ／ｄ上皮生长因子（ＥＧＦ）皮下给药促进大白鼠小肠缺血再灌注损伤后粘膜的修复，观察细胞的增殖。方法ＳＤ雄性大白鼠６０只，阻断肠系膜上动脉后去除阻断为小肠缺血再灌注损伤模型，随机分为对照组（Ａ）、皮下放置不含ＥＧＦ缓释片组（Ｂ）和放置ＥＧＦ缓释片组（Ｃ）。观察各组０时（缺血６０分钟再灌注１２０分钟后）、第２、４、８天组织学改变，并进行粘膜细胞ＤＮＡ流式细胞仪分析。结果组织学检查示Ｂ组再生绒毛较Ｃ组短小、稀少。肠粘膜细胞经流式细胞仪ＤＮＡ分析，结果表明４８小时修复，Ｃ组Ｓ期细胞（１３．０２％±１．３９％）较Ｂ组升高更为明显（ＣｖｓＢＰ＜０．０５），损伤修复后第４、８天，Ｂ组细胞增殖已趋于正常，与Ａ组比较Ｐ＞０．０５，而Ｃ组上皮细胞增殖仍居高不下，较Ｂ组和Ａ组均有统计学意义（Ｐ＜０．０１）。结论ＥＧＦ不仅可促进缺血再灌注后小肠粘膜的再生修复，同时有助于消化吸收功能的早期恢复。
Objective This experiment used 10us / d epidermal growth factor (EGF) subcutaneously to promote mucosal repair after intestinal ischemia / reperfusion injury in rats and observed cell proliferation. Methods Sixty male Sprague-Dawley rats were divided into control group (A), subcutaneous injection of EGF-free (B) group and EGF Sustained release tablets group (C). Each group 0 (ischemia 60 minutes after reperfusion 120 minutes), 2, 4, 8 days histological changes, and mucosal DNA flow cytometry. Results Histological examination showed that the regenerated villi in group B were shorter and sparse than those in group C. The intestinal mucosal cells were analyzed by flow cytometry. The results showed that the cells in group C (13.02% ± 1.39%) were more obvious than those in group B (CvsBP <0.05) after 48 hours of repair. The damage On the 4th and 8th day after repair, the cell proliferation of group B tended to be normal, compared with that of group A (P> 0.05), while the proliferation of epithelial cells of group C remained high, which was significantly higher than that of group B and group A ( P <0.01). Conclusion EGF can not only promote the regeneration of intestinal mucosa after ischemia-reperfusion, but also help the early recovery of digestion and absorption.