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目的研究幽门螺杆菌(HP)培养滤液对SGC7901胃癌细胞表达hTERT、Bcl-2蛋白的影响。方法在HP培养滤液诱导前后,采用流式细胞仪检测SGC7901胃癌细胞hTERT、Bcl-2蛋白的表达。结果对照组SGC7901胃癌细胞表达hTERT和Bcl-2蛋白的阳性细胞数和荧光指数显著低于经HP培养滤液诱导的SGC7901胃癌细胞组(P<0.05)。经HP培养滤液处理的SGC7901胃癌细胞24、36、48 h表达hTERT和Bcl-2蛋白的阳性细胞数和荧光指数呈递增趋势,且相互间有显著差异(P<0.05)。结论HP感染不仅通过激活端粒酶促进细胞永生化,而且通过促进Bcl-2表达使细胞凋亡减少,进而促进胃癌的发生、发展,这可能是HP感染致癌的重要机制之一。
Objective To study the effect of Helicobacter pylori (HP) culture filtrate on the expression of hTERT and Bcl-2 protein in SGC7901 gastric cancer cells. Methods Flow cytometry was used to detect the expression of hTERT and Bcl-2 protein in SGC7901 gastric cancer cells before and after HP filtrate was induced. Results The number of positive cells and fluorescence index of hTERT and Bcl-2 protein expression in SGC7901 gastric cancer cells were significantly lower than that of SGC7901 cells induced by HP culture filtrate (P<0.05). The number of positive cells expressing hTERT and Bcl-2 protein and the fluorescence index of SGC7901 gastric cancer cells treated with HP culture filtrate showed an increasing trend at 24, 36, and 48 h, and there was a significant difference between them (P<0.05). Conclusion HP infection not only promotes cell immortalization by activating telomerase, but also promotes the apoptosis of gastric cancer by promoting the expression of Bcl-2, which may promote the occurrence and development of gastric cancer. This may be one of the important mechanisms of HP infection.