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为了解人类乳头瘤病毒基因（ＨＰＶ－ＤＮＡ）６、１１及１６型及ｐ５３蛋白在食管鳞癌中的作用及其与病人预后的关系，对６４例食管鳞癌标本用原位分子杂交法检测了ＨＰＶ－ＤＮＡ６、１１和１６型表型；用免疫组化法检测了ｐ５３蛋白。结果发现：６４例食管鳞癌中ＨＰＶ－ＤＮＡ总的检出率为６２．５％，感染率较高，但ＨＰＶ６、１１型和１６型之间的检出率无显著差异。ＨＰＶ６、１１型的阳性率和肿瘤细胞分化程度有关，高分化者检出率高，低分化者检出率低。ＨＰＶ１６型的检出率在淋巴结转移组中比无转移组高；在ＴＮＭ分期Ⅲ、Ⅳ期的检出率比Ⅱ期者高；病人存活１年以内组中的检出率比存活３年以上者高。结论表明感染ＨＰＶ１６型者预后不佳；ＨＰＶ与ｐ５３双阳性者预后不佳。
To understand the role of human papillomavirus gene (HPV-DNA) 6, 11 and 16 and p53 protein in esophageal squamous cell carcinoma and its relationship with the prognosis of the patient, 64 specimens of esophageal squamous cell carcinoma were detected by in situ hybridization. HPV-DNA 6, 11 and 16 phenotypes; p53 protein was detected by immunohistochemistry. The results showed that the total detection rate of HPV-DNA in 64 cases of esophageal squamous cell carcinoma was 62.5%, and the infection rate was high, but there was no significant difference in the detection rate between HPV 6, 11 and 16 types. The positive rates of HPV types 6 and 11 are related to the degree of differentiation of tumor cells. The detection rate of highly differentiated persons is high, and the detection rate of poorly differentiated persons is low. The detection rate of HPV16 was higher in the lymph node metastasis group than in the no metastasis group; the detection rate in the TNM stage III and IV was higher than that in the phase II; the detection rate in the patient within one year of surviving was higher than the survival rate for more than 3 years. High. Conclusions suggest that the prognosis of HPV16 infection is poor; HPV and p53 double positive patients have poor prognosis.